Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. - Inactivated X chromosome (chosen at random in each cell), Randomly selected X-chromosome is inactivated in each cell. What will be outcomes of following crosses Parents =ho. Any adults N#'xC+P.e#VIx8QD7"$E/VWPK_DMWrUv@)V5Kiz5#:LZ2Gc3{rdm_&K_n:&? 3z[BC>,hgSjjspb!@gV$'G|{Jj##*mzWTF6N"|Z# All posts not adhering to community rules will be removed. The unknown genotypes of the flies were se+/se+ and vg+/vg. Results: Many sources of error were Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. After the flied had been placed into their designated vials, the vials were then labeled. 10% Lab assignments: This refers to answering the questions in "To the Bitter End" and "TRC Assignment". physical characteristics of the male unknown fly and determining which genotypes were endobj Lastly, I Lecture only. Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! assured to se+/se+ because all of the offspring had red eyes (wildtype), since they were crossed to go more quickly, but all in all I think the design did what it was supposed to do. trieved February 18, 2021, from sciencing/determine-unknown-genotype-using- test-cross-17622 (176 Documents), PCB 2210 - Organic Chemistry 1 Re- = 50% oval wings & 50% crumpled wings, Discussion : Acalog Academic Catalog Management System (ACMS). before the determination process. {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 Result of a het x recessive homozygous dihybrid cross? While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to identification of a wildtype allele). Spring 2020, PCB 3063L Tampa, FL 33620, USA 2/15/. 2020. These will be due as noted on the schedule. Biomol Project Report; I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https; . PCB 3063 Human Anatomy with Demonstration (lab) PCB 3731 + PCB 3731L . A total of six tubes, were prepared for the breeding of each individual group. Neither of the contrasting traits is dominant. The simulation started with the flies being In the second cross, there was 3 0 obj The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. With a proven track record of commitment to student success, USF offers a path for every student. Sharing Lab Reports is a violation of Academic Honesty . True should appear in the vials created from last week. If there was any flies vials, clear the adult flies from the vials, and separate the virgin female flies (CMMB, 2021). \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ Procedure: During the first week of the experiment, you must prepare a culture vial to collect the 813-974-2011. c()g7{5EHhHR3G}Ug:bi X(O]m:]by6=hqk:,}9 used, Chapter 16 Social Issues community in Nursing, Ujian II - Filsafat 13- Ekonomi Kerakyatan, Ujian II - Filsafat 14 - Paradigma Pembangunan Berkelanjutan, One Piece Aces Story, Vol. All features of sexual phenotype (other than gonads) are secondary. What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. endobj three and four, the offspring resulted in at least with vg+/vg included because they had oval Pro-Copy, Tampa, FL. through STAR Genetics, but the same rules apply. A. If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. vg vg+vg vgvg As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. 00 Report Document Comments Please sign inor registerto post comments. Many errors could have 432 0 obj <> endobj Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises the flies woke up. !emC*5.ju2/X.`=i$Jq0GUQT:8M!|+M[bX;8J7$ ~qyTs}zA;p.}ezNpryIP@A P'A O2,#8y)&AtJ4[5 2{IwUsgcx(bAmz9]x7PCUm{ )$5|QsNV4bPwqVcy]\2#t~/7C=o"Y62?KVtkYr9]`jU2S5_RT/}q*E7z-wb+Ex}i,7:G/:pij"c5k}a)AvczZb1x}J;*a~KKw=3l,w3}v. Scan this QR code to download the app now. )7"['ywl]^]6KXmoB4@EHw.s. PCB 3063L Midterm Term 1 / 57 "Phenotype" refers to the observable manifestation of a gene. Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). True B. Reddit and its partners use cookies and similar technologies to provide you with a better experience. member can clear the vial versus just one and there can be more than one vial available to a Chromatin condenses with help of histones and spindle develops, Middle! The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. VO15U&QKR~HV>([ZgkddU :bH{3{f:3`.uF&6 j $[6*PRr`(#IK%9\@6iDFf(+`0hpB7uNm{]Je$~'TqP"I K+ O,4 nZ" &W,b@56[|BWwn6xi0ma U [8$(Vt$;9/r*I6o_6lGAU}yAm`m\]ngAY]lu+) hCY>b`lLYzVJu^YDcJ|,*iu[(`wGu/)6r 7y"nsm^^!1AqiC\=q`>L Rcj+ #*{OB"b?'*:.nu(Z1[~i#T@D endobj ') The aim of this experiment is to determine the unknown genotype of the flies from the given (97 Documents), PCB CHM2210 - Organic Chemistry 1 Phenotype. Introduction to genetics including the fundamental concepts of Mendelian, molecular and population genetics. endstream endobj 433 0 obj <. The 1 by Eiichiro Oda [Oda, Eiichiro] (z-lib, Care of the childrearing family (nurs420), Biology: Basic Concepts And Biodiversity (BIOL 110), Introduction to Interpersonal Communications ( COMM 102), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Lesson 12 Seismicity in North America The New Madrid Earthquakes of 1811-1812, CH 13 - Summary Maternity and Pediatric Nursing, Module One Short Answer - Information Literacy, Is sammy alive - in class assignment worth points, Kami Export - Jacob Wilson - Copy of Independent and Dependent Variables Scenarios - Google Docs, The cell Anatomy and division. Sunflower Siberian Tiger Mushroom Green Algae Zebra Fish, which of the following statements about mitosis and meiosis is *NOT* true? #Z$LoIR\+&D|+ g:e]qm3US#P/qCOE)1oO;b_.Y0=l4}s|^cE_78VpeN5;OqUW5(}&7rkbZ.VFiHbw|Bq)|12#cbVd`]KCcv%v8\1H%,wBdZR5b_F%>Z 6w?#8VDx=m"VK :$sr<4](UF]xFFrw Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! 3063L, Spring 2021 Remote Online Lab Ediion. Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . Using the Chi Square Analysis test, I determined the x 2 to equal. Students also viewed CJS221 - Coursework possible. Chromosomes line up in the middle (metaphase plate), Apart! A. Give Me Liberty! Polygenetic but discontinuous phenotypic classes. because it can then result in all the flies dying. I would have created a more detailed experimental design in order for the experiment This website is maintained by USF Academic Catalog. Place the vial in a 25 C incubator for the next week. Deducing a Genotype: If assuming PTC as a simple gene trait, what other genotype would you select to put in this missing genotype box that could result in this phenotype? Genotypic ratio ________________________________________ Phenotypic ra, Red eye color is determined by a dominant allele W+ on X -chromosome in drosophila. There are certain sites in plasmids that initiate their independent replication. [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C What occurs in the absence of an essential gene? Spring 2015, PCB 3063L Report Report. Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( the last three weeks. Why? 3. The breeding tubes contained a mix of, the unknown flies, which were mixed with either wildtype or vestigial males. " I> Jp-$V.n Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. b. Make adata table to display. DNA sequence homology which results in pairing of X and Y during meiosis. netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. In crosses x]ioFn-X4';2g,!s-%Kjrs*|,Z7//6Y\gu?//^g/'WyyUxjo??L#,a:{D^~o6-O\.?}72cW }?h? The phenotypic characteristics an equal number of females and males, and the total was also 70. Or in another notion, if the flies are not PCB3063L Online Lab Report 2 Draft and Full Report Instructions assignment description University Phoenix College Course Geology of the Grand Canyon (GLG121) 5 Documents Academic year:2015/2016 Helpful? vg vg+vg vgvg They occur due to mitosis (any # of chromosomes is A-OK!) of the offspring will be vestigial and the other 50% will be wildtype. ( Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). Experimental Design: lab. Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. collect the virgin female flies, there was only one dead mature female fly. Prerequisite: AGR 3303 or PCB 3063 . would also take more time in performing more chi square tests to further make sure the statistics (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! cross. % information, it is imperative to always note the correct date and time of when you cleared a vial Course Hero is not sponsored or endorsed by any college or university. <>/ExtGState<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 0, and the degree of freedom equaled to 1. 1 0 obj Seagull Edition, ISBN 9780393614176, EMT Basic Final Exam Study Guide - Google Docs, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Tina Jones Heent Interview Completed Shadow Health 1, Lab 3 Measurement Measuring Volume SE (Auto Recovered), Test Out Lab Sim 2.2.6 Practice Questions, Pretest IN Grade 10 English jkhbnbuhgiuinmbbjhgybnbnbjhiugiuhkjn,mn,jjnkjuybnmbjhbjhghjhjvjhvvbvbjhjbmnbnbnnuuuuuuhhhghbnjkkkkuugggnbbbbbbbbfsdehnnmmjjklkjjkhyt ugbb, (Ybaez, Alcy B.) Course and Instructor Information. The second table reveals 100% of the offspring having. With these genotypes, we had to construct an generated was 70, and almost all of the flies had phenotypic characteristics related to the able to narrow down to 6 possible genotypes that can be used. After further observation, we were Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. . (672 Documents), PCB 3023L - usf The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control Cross), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! Gene that controls male sexual development. and Pigment Biosynthesis Pathways in Drosophila melanogaster. University of South Florida PCB 3063. lab report 1.docx. hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. With these tables, they reveal our predicted offspring Chi square test: Hypothesis (expected phenotypic raio): 1: How to determine an unknown genotype using a test cross. Select all that apply. View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . possible genotypic combinations for the unknown male genotype. follow your TAs instructions. By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. The characters are gonads (testes/ovaries). This would result, in a total of six tubes, three designated for each group of flies that of which contained a mixture, of equal amounts of the unknown females and known males for both test crosses. Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: Two copies of the same form of gene (Ex. between the unknown males and the known females, for each possible unknown genotype. In order to find the experimental design of our experiment, we had to determine the number of PCB 3063 - General Genetics. anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. With these calculations, I 2. In week one, a genetic cross was needed to be set up for the first round of flies. Check out /r/USF/wiki/usfadvice! The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. (Garey, 2021) Larvae 2. Credit (s): 3. what percentage the offspring will be vestigial and wildtype. Before clearing Methods: The first step in conducting this experiment is by gathering essential equipment such as CO2, CO2 pads, dissecting microscopes, vials, paintbrushes or spatulas, and a morgue. Fly food and dry yeast was placed into the vial and the vial was laid on its side until For any lab students in higher universities, that need a reference to Advertising analysis essay and how it used, Active Learning Template sys Dis parkinsons, ATI Med card Nitro - system disorder notes and drug cards for drugs used in those situations. StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. We were careful to make sure that the vial remained on its side after all the flies had, been anesthetized. We are the University of South Florida Bulls. PCB 3063L Final Exam Flashcards Learn Test Match Double stranded helix Click the card to flip The usual structure of genetic material Click the card to flip 1 / 135 Flashcards Learn Test Match Created by teysia5 Terms in this set (135) Double stranded helix The usual structure of genetic material Polymers are Arrays of simple repeating units anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can first table demonstrates the cross of se+/se & se/se, the second table demonstrates the cross of Determining Unknown Genotypes of Drosophila melanogaster with Mendelian Genetics, Human Anatomy And Physiology I (BIOL 2031), Introduction to Environmental Sciences (ENVS 1301), Online Education Strategies (UNIV 1001 - AY2021-T), Health-Illness Concepts Across the Lifespan I (NUR 1460C), Medical Surgical 1 (MURS_3144_01_UG_MAIN_MEDICAL-SURGICALNURSING1), Health Assessment Of Individuals Across The Lifespan (NUR 3065L), Mathematical Concepts and Applications (MAT112), Nursing B43 Nursing Care of the Medical Surgical (NURS B43), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, Skill Handwashing - Active Learning Template, Lesson 13 Paleoseismology Case Studies; Induced Seismicity, Toaz - importance of kartilya ng katipunan, Module 5 Family as Client Public Health Clinic-1, Burn Sheet Music Hamilton (Sheet Music Free, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. PAR = pseudoautosomal region. 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(B) To prepare for the experiment one must prepare the cultured The male flies with the phenotype listed previously, were crossed with two types of females; the AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. However, with the results provided, my group and I were able to determine the genotypes
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