Gut 63, 588597, doi: 10.1136/gutjnl-2013-304636 (2014). In addition, fewer female mice achieved surgical anesthesia, suggesting a narrow therapeutic window for intraperitoneal KX in female mice. sharing sensitive information, make sure youre on a federal PubMed 2020 May 9;12(5):439. doi: 10.3390/pharmaceutics12050439. Quantitative data are presented as the meanSD. While the DiR labeled cells showed very different cell distribution. Six leukemia patients with Crohns disease treated with allogeneic marrow transplantation and four of five patients remained CD free 6 to 15 years after transplantation10. Little is known about neuronal cell death in mouse models of diabetic retinopathy. Gastroenterology 132, 944954, doi: 10.1053/j.gastro.2006.12.029 (2007). Briefly, colonic segments were homogenized at 50mg/mL in phosphate buffer (50mM, pH 6.0) with 0.5% hexadecyltrimethylammonium bromide. Here, three different MSCs delivery routes: intraperitoneal (IP), intravenous (IV) and anal injection (AI) were compared on DSS-induced colitic mice model. volume6, Articlenumber:30696 (2016) PubMed Central Novel mechanism for mesenchymal stem cells in attenuating peritoneal adhesion: accumulating in the lung and secreting tumor necrosis factor alpha-stimulating gene-6. MathSciNet Opioids can produce peripheral analgesic effects by activation of opioid receptors on sensory nerves. eCollection 2021. 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. Bookshelf Stem cell transplantation: the lung barrier. The fecal occult blood test on the day 3 also showed nearly complete absence of occult blood in IP group. MSCs has been well characterized of producing a range of modulatory cytokine, which inspires the designation of these cells as injury drugstore44. Lopez-Cubero, S. O., Sullivan, K. M. & McDonald, G. B. Zhang, B. et al. ADS Mouse insulin gradually decreased and was no more detectable 13 weeks after STZ injection. [citation needed]. 4C). A modified human recombinant type 5 adenovirus . Makhija, S. et al. Clinical Ovarian and Other Gynecologic Cancer 5, 2426, doi: 10.1016/j.cloc.2011.08.001 (2012). Macrophage Reverse Cholesterol Transport | Circulation Intraperitoneal Drug Delivery for Ovarian Cancer: Why, How, Who, What 2021 Jul 23;2021:2303417. doi: 10.1155/2021/2303417. The levels of immunomodulatory cytokines were compared by real time-PCR and ELISA. Epidemiology and natural history of inflammatory bowel diseases. Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. IP injection showed better amelioration of colitis and may be an ideal delivery way for MSCs in IBD therapy. How to cite this article: Wang, M. et al. After antigen retrieval, the endogenous peroxidase activity was blocked by 1% H2O2 for 10min. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Intraperitoneal chemotherapy for peritoneal metastases: an expert opinion. This study compared intraperitoneal injections, intravenous injections, and anal injections. The Scheme (Fig. Unauthorized use of these marks is strictly prohibited. Stem cells and development 21, 21892203, doi: 10.1089/scd.2011.0674 (2012). However, human insulin . Intraperitoneal foreign bodies such as suture material, clips, and mesh also contribute to adhesion formation. The physiologic characteristic of the peritoneal cavity not only helps remove toxic metabolites from the body, but also provides a useful portal of entry in the body for several pharmacological agents. Lee et al. The IP injection showed longer colon, with mean length of 8.24cm compared with 6.90cm in AI group (P=0.0057) and 6.14cm in IV group (P<0.001; Fig. injections. Essentials of Human Nutrition - Jun 13 2022 . & Correa, D. The MSC: an injury drugstore. There was a dose-dependent decrease in AGT activity in liver tissues of CD-1 mice to 24% of basal levels after four hourly intraperitoneal injections of O 6-methylguanine (110 mg/kg). PubMed Central PubMed Inflammatory bowel diseases (IBD) is a broad term that involves chronic inflammation of all or part of the gastrointestinal tract. The holder retains the arms of the animal and tilts the head lower than the abdomen to create optimal space in the peritoneal cavity. Qualitatively, the existence of non-portal drainage was demonstrated by . Injection into egg 1 month 20 doses 1 dose 1 month 1 month Once 3.0 mL/kg 5 mL/kg 10 mL/kg 2.5 mL/kg 1 mL/kg O.^L/g Well tolerated Well tolerated Well tolerated Well tolerated Well tolerated Less mortality 1 \Jg Increase in mortality, decreased activity during righting reflex, running time, visual discrimination, and olfactory aversion test FoxP3+ cells were greatly increased in MSCs therapy. Google Scholar. More recently the IP route has been used for chemotherapy in patients with intra-abdominal malignancies, i.e. In 1996, intraperitoneal (IP) administration of cisplatin plus intravenous (IV) cyclophosphamide proved superior to both drugs given intravenously at the same doses-which, at the time, was the standard treatment in the United States. . PubMed The primer sequences used were listed in Supplementary Table S1. Kurtz, A. Mesenchymal stem cell delivery routes and fate. Key points to note. Normal mouse IgG was used as a substitute for the primary antibody as negative control. Gonzalez, M. A., Gonzalez-Rey, E., Rico, L., Buscher, D. & Delgado, M. Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Collagen deposition is another indicator for wound healing, however, extensive collagen deposition may hamper the gut reconstitution14. Ballinger AE, Palmer SC, Wiggins KJ, Craig JC, Johnson DW, Cross NB, Strippoli GF. n=3. MSCs has been found to directly differentiate into end-stage cells to augment bone damages and repair cartilage defects38,39. Cells, tissues, organs 169, 1220 (2001). 2, Haidian District, Beijing 100193, China. Garcia-Bosch, O., Ricart, E. & Panes, J. and H.H. Proliferation index was defined as percent of Ki-67 positive nuclei within the random visual scope. In various preclinical models, saroglitazar has been shown to prevent & reverse symptoms of NASH. Not only in animal studies, some clinical trials using MSCs to treat osteogensis imperfect (OI) in GvHD, also showed less than 1% cell were detected in target organ18,19,20. In addition, MSCs showed inhibitory effects on proliferation of memory T-cells by direct cell contact52. The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: By systemic evaluation of epithelium and lymphocyte infiltration, the IP injection group showed the relatively lower severity score compared with AI group (P=0.034) and IV (P=0.002), respectively. Real time-PCR and ELISA also demonstrate lower TNF- and higher IL-10, TSG-6 levels in IP group. However, no significant TSG-6 difference was found between AI and IV groups. From the overall appearance, the hyperemia and edema of the colon in all MSCs treatment groups became milder than the DSS control. [17] Contrast agents were administered intraperitoneally instead of intravenously to avoid errors and challenges. RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio Rad, Hercules, CA). [1] Fluids are injected intraperitoneally in infants, also used for peritoneal dialysis. Thus, we have succeeded to produce patient-like tumor colonization of the peritoneal cavity and hepatic metastasis with the human EAC cell line OE19 by intraperitoneal injection in SCID mice. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Refractory Malignant Ascites. Google Scholar. To varying degrees, all the three MSCs delivery ways could ameliorate DSS-induced colitis. Gastroenterology 136, 978989, doi: 10.1053/j.gastro.2008.11.041 (2009). : 1950) 180, 15981608 (2008). The https:// ensures that you are connecting to the These complications include catheter infection, physical damages to intra-abdominal structures, such as vaginal vault perforation, bladder erosion and bowel perforation58 and direct drug toxicity to non-specific abdomen organ (e.g. The overall therapeutic factors, MSCs migration and targeting as well as local immunomodulatory cytokines and FoxP3+ cells infiltration were analyzed. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. . Other than preformed at the site of injury, MSCs has showed ability to exert their therapeutic effects distally. PubMed Central PubMed However, most of these studies were focus on its therapeutic effects or mechanisms. For some severe cases, IBD may lead to the life-threaten complications5. Study on pharmacokinetics-Study on pharmacokinetics-Innostar [14] The symptoms of this included impairment of cognitive and psychomotor abilities. Respir Res 15, 118, doi: 10.1186/s12931-014-0118-x (2014). It is more often applied to animals than to humans. Tuning the Physicochemical Characteristics of Particle-Based Carriers for Intraperitoneal Local Chemotherapy. Bioengineered. Collagen deposition limits immune reconstitution in the gut. Generally, the FoxP3 expression was elevated in MSCs treatment (Fig. Very few reports pay attention to factors applied in the clinic, such as injection or exposure routes, which has highly impact on stem cell therapeutic efficiency for IBD12. Stem cells (Dayton, Ohio) 24, 386398, doi: 10.1634/stemcells.2005-0008 (2006). IP injections are used for a variety of reasons: The peritoneum provides a large surface area for absorption of drug (compared with intramuscular (IM) or subcutaneous); can thus inject a larger fluid volume. For example, discharged accompany with stool or cleared out by lung microphage. It was concluded in a review article that utilizing IP injections to administer drugs to laboratory rodents in experimental studies is acceptable when being applied to proof-of-concept studies.[15]. PubMed Central Arthritis and rheumatism 56, 11751186, doi: 10.1002/art.22511 (2007). official website and that any information you provide is encrypted Interaperitoneal Administration of lpha-Tocopherol Loaded Nanoparticles Improves Ischemia-Reperfusion Injury in Rat Ovaries Torsion and Detorsion Model. Best way for mesenchymal stem cells transplantation for colitis. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). PDF Guidelines for the Research Use of Adjuvants - National Institutes of Slider with three articles shown per slide. [20] There needs to be more research done to determine appropriate dosing and combinations of these drugs to advance intraperitoneal drug delivery. Bethesda, MD 20894, Web Policies Sci Rep 6, 30696 (2016). To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Wang, M., Liang, C., Hu, H. et al. In this study, intraperitoneal (IP) injection was used to mimic the human administration route. World journal of gastroenterology 20, 1822818239, doi: 10.3748/wjg.v20.i48.18228 (2014). Side effects. In the meantime, to ensure continued support, we are displaying the site without styles Gastrointestinal Absorption of Insulin-like Growth Factor All authors have reviewed the final version of the manuscript and approve it for publication. In humans, numerous plasma molecules prevent or modulate cell stimulation by microbial products, either by neutralizing the agonists (eg, bactericidal permeabilityincreasing protein, . In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Tests were read by an independent observer (Cong Liang) and scored 05 as shown by the color indicators provided by the manufacturer. In vivo clearance of free DiR in healthy mice was showed in Supplemental Figure S4. Available at: http://www.cdc.gov/ibd/ (Accessed: 7th April 2016) (2014). It is more often applied to animals than to humans. and arterial blood in humans during peritoneal dialysis therapy with 4 mmol 5-fluorouracil. Depletion of O6-alkylguanine-DNA alkyltransferase activity in mammalian [19] It was concluded that IP administration should not be used over intravenous therapy due to high radiation absorption in the intestines. Intraperitoneal Injection. Injections are given along the midline caudal to the umbilicus or low on the right lateral abdomen. The DiR dye alone was served as control. After intraperitoneal injection of macrophages labeled with [3 H]cholesterol, the tracer can be detected in the plasma, liver, . 3) and the IV group preserved fewer cell number. However, doubts exist over the use of Fe 3 O 4-nanoparticles in human beings. It causes irritation of the eyes, skin, mucous membranes, and upper respiratory tract. Injection Techniques, Restraint, & Handling for Mice and Rats It also shows how easily substances are absorbed through the peritoneal cavity after an IP injection. Article The GFP+ MSCs were found at inflamed colon in both IP and AI groups 24h after MSCs injection. Here, we also compare the expression of FoxP3, which is frequently used to quantitate Treg cells. Google Scholar. There are few accounts of the use of intraperitoneal injections prior to 1970. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. Article Wang, N. et al. Intraperitoneal Injection in the Mouse - Research Animal Training In contrast, the DSS control showed 15.1% Ki-67+ cells/crypt. government site. Intradermal. Dosage: For intravenous (preferred), intracardial, intrapleural, or intraperitoneal injection. Note where each injection is made and subsequent injections should be made in slightly different spot than the previous injection. The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. evaluated the therapeutic efficacy of different MSCs sources and delivery routes in experimental emphysema37. Intraperitoneal injection | Detailed Pedia -Lactalbumin is an example of a "retired" self-protein because its expression in normal . The IP and AI groups showed mean 50.4% and 46.7% Ki-67+ cells/crypt respectively. Comparison of the Effects of Intraperitoneal Injection with Carbon Google Scholar. Reduced MPO activities were identified in all MSCs groups, of which IP injection showed lower MPO activity (P=0.001vs. IV and P=0.0158vs. AI; Fig. PubMed Accessibility In our study, we also identify MSCs existence in nearby MLN. Available at: http://ctep.cancer.gov/highlights/20060105_ovarian.htm (Accessed: 7th April 2016) (2006). Language links are at the top of the page across from the title. 2A). In addition, the effects of different dosing modalities (e.g., oral administration and intraperitoneal injection) on the . Like pharmacological treatment, the cell dosing might also be an important issue for MSCs therapeutic success21. The excitation/ emission spectrum of DiR is in the near infrared range (excitation 750nm and emission 782nm). 2B). Increased initial cell dose ensures an increased number of cells that reach to the injury site. At day 5, mice were randomized and injected with MSCs (1106, 200L in volume) by three delivery routes (IP, AI and IV). Hsiao, S. T. et al. For example, if using a dose of 1 U kg -1 , a concentration of 0.2 U ml -1 is suitable. Sminia, T., Soesatyo, M., Ghufron, M. & Thepen, T. The migration of peritoneal cells towards the gut. Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injury. [4] In 1964, a study delivered chemical agents such as acetic acid, bradykinin, and kaolin to mice intraperitoneally in order to study a "squirming" response. Integrative genetic analysis identifies FLVCR1 as a plasma-membrane PDF Intraperitoneal (IP) Injection in Rats and Mice SOP Afterwards, the samples were incubated with Ki-67 (1:200; Santa Cruz) or FOXP3 (1:100; Antibodies-Online, Aachen, Germany) overnight at 4C. An example of when intraperitoneal injections are not ideal is given in a study where the best route of administration was determined for cancer biotherapy. One example is using ultrasound-navigation for puncture. In consistent with Scaldaferris work, the increased TSG-6 after MSCs transplantation was also identified. Ex-vivo study was carried out at 1 day post-injection. Ray, A. In this study, the therapeutic efficiency of three different MSCs delivery routes (IP, AI and IV) were compared on DSS-induced colitis mouse model. Analysis of tissues following mesenchymal stromal cell therapy in humans indicates limited long-term engraftment and no ectopic tissue formation. Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? prepared Figure 5. The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. GTTs and ITTs in mice: simple tests, complex answers - Nature There is a little fluid in between the peritoneal lining and your organs. Because of the injection of cannabis, the patient was predicted to have some level of THC in the blood from absorption. Horwitz, E. M. et al. Stem Cell Res Ther 3, 51, doi: 10.1186/scrt142 (2012). seem to have found the key drug of MSCs in the treatment of colitis, TSG-647. Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. They are one of the few ways drugs can be administered through injection, and have uses in research involving animals, drug administration to treat ovarian cancers, and much more. A . Our work used marrow-derived MSCs but not adipose-derived ones. humans have been associated with clinical pain, inflammatory lesions, and abscess formation in . Vilquin, J. T. & Rosset, P. Mesenchymal stem cells in bone and cartilage repair: current status. and S.J. An official website of the United States government. Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. In COX-2 blocking control experiments, mice received FA-NPs (0.5 mg/kg) 1 h after an intraperitoneal injection of celecoxib (10 mg/kg) to block binding of FA to the COX-2 active site. In IP group, cells were found accumulation at the epithelium; In AI group, cells were mainly in the lumen; In IV group, cells were fewer and located in lamina propria. [10] The results depicted that ketobemidone consumption was significantly lower when patients controlled anesthetic through IP. & Gratwohl, A. Intraperitoneal injections are typically made in the lower right or left quadrant of the hamster's abdomen, not on the midline. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. and transmitted securely. Shariati M, Zhang H, Van de Sande L, Descamps B, Vanhove C, Willaert W, Ceelen W, De Smedt SC, Remaut K. Pharm Res. NIR fluorescent dye, 1, 1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR; DiIC18(7), Molecular Probes, Invitrogen, Carlsbad, CA) was used for labeling the cells as previously reported65. We even tried 1108 cell/mouse in IP and AI group and no immediate death was occurred. Wang Min, Liang Cong and Hu Hao contributed equally to this work. Human bronchial epithelial cells (BEAS-2B and 16HBE) were purchased from Shanghai Zhongqiao Xinzhou Biological Co., Ltd, and cultured in a complete medium supplemented with growth factors and .1% penicillin-streptomycin. 2020 Jun 3;37(6):119. doi: 10.1007/s11095-020-02818-8. The Journal of clinical investigation 117, 258269, doi: 10.1172/jci29159 (2007). Study showed that genetically modified MSCs to increase CXCR-4 expression will lead to an increase of MSCs migration to intestinal with radiation enteritis and then resulted in disease improvement24. von Bahr, L. et al. Intraperitoneal Route of Drug Administration: Should it Be Used in Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6. Estes, J. et al. Yu-Lung Chang, Huei-Yu Lo, Chin-Kan Chan, Xing-hua Pan, Qing-qing Li, Guang-ping Ruan, Hongsheng Yang, Rui Feng, Shenghong Zhang, H. R. Wardill, C. E. M. de Mooij, N. M. A. Blijlevens, Suze A. Jansen, Edward E. S. Nieuwenhuis, Caroline A. Lindemans, Scientific Reports From two streams of research, experimental and clinical, stem cell therapy showed promising results. Mice were made diabetic by intraperitoneal injection of streptozotocin, which led to a 2- to 2.5-fold increase in plasma glucose. Importantly, we identified different migration fates of MSCs and traced their ultimate location of three administration ways for the first time.
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